Chemical synthesis... uhmm, something that not easy to do. We don't have eyes that could see any molecular reactions happened, we don't know if our expected product has already formed or not, if our product really exist in our beaker. Therefore, we need "a helping eyes" that could visualized that unseen thing. There's one popular method that could be used as our "helping eyes", Thin Layer Chromatography.
Thin layer chromatography (TLC) is widely used method in chemical analysis because it is easy to perform, it could be used in qualitative and quantitative analysis, also it is relatively not expensive yet effective. The basic thing happened in TLC is separation based on polarity, affinity of compound to the plate (stationary phase) and the solvent (mobile phase).
Does every compound could be "seen" using this method? I guess so, but we have to know the suitable system to "see" it. For example, to see compounds that have conjugated double bond, use stationary phase that added with fluorescence agent then use UV lamp to see the florescence. If not.. what would you expect?
Okay, to know more about TLC, I would like to recommend a very interesting book, Thin Layer Chromatography : A Modern Practical Approach (Rsc Chromatography Monographs)
. As its title you can get practical things about TLC, let say how to choose the best sorbent material regarding the sample compound, how to cut TLC plates (well.. many people don't know how to perform it..), how to do TLC modification. This book really suitable for those who don't know much about TLC (well, just like me :P). I got a problem in doing fatty acid analysis, just got informed that AgNO3 impregnated TLC could help. The further information about it I got from this book.. Happy.. really happy. Well, let us know from the expert, read Thin Layer Chromatography : A Modern Practical Approach (Rsc Chromatography Monographs).
Very recommended book for knowing more about Thin Layer Chromatography :D
Thin layer chromatography (TLC) is widely used method in chemical analysis because it is easy to perform, it could be used in qualitative and quantitative analysis, also it is relatively not expensive yet effective. The basic thing happened in TLC is separation based on polarity, affinity of compound to the plate (stationary phase) and the solvent (mobile phase).
Does every compound could be "seen" using this method? I guess so, but we have to know the suitable system to "see" it. For example, to see compounds that have conjugated double bond, use stationary phase that added with fluorescence agent then use UV lamp to see the florescence. If not.. what would you expect?
Okay, to know more about TLC, I would like to recommend a very interesting book, Thin Layer Chromatography : A Modern Practical Approach (Rsc Chromatography Monographs). As its title you can get practical things about TLC, let say how to choose the best sorbent material regarding the sample compound, how to cut TLC plates (well.. many people don't know how to perform it..), how to do TLC modification. This book really suitable for those who don't know much about TLC (well, just like me :P). I got a problem in doing fatty acid analysis, just got informed that AgNO3 impregnated TLC could help. The further information about it I got from this book.. Happy.. really happy. Well, let us know from the expert, read Thin Layer Chromatography : A Modern Practical Approach (Rsc Chromatography Monographs).Very recommended book for knowing more about Thin Layer Chromatography :D
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