I've been told that some (or maybe all) of you don't understand about the note that I wrote in your microbiology discussion paper. Yeah, I know it's late as you have to submit the report tomorrow :P. Hope this note can help you.
- The inhibtion zone diameter dimension changed to mmx10. Well, I don't know either the exact reason. I think it's only statistical reason. You used the caliper to measure the inhibition zone diameter and you got the measure for example 2.45 cm. The decimal shows the degree of accuracy. On the other side, biological assay has low accuracy (because of the biological variation). Because of that, you need to convert the diameter to mmx10 that is 100 times than the diameter measured to delete the "point" sign.
- Generate callibration curve. The calibration curve generated from YL, YH value and log of concentration. After that, find out the line equation, you can use calculator or Microsoft Excel. Most of you reported the same wrong equation, without asking anyone of you I already know the reason why -_-'.
- The uses of this experiment for diagnostic purpose. Well, I know diagnostic things are for community and clinical pharmacy, not for you that majoring pharmaceutical science and technology but there's no wrong to know more isn't it? I guess you don't have any idea of this part.. so I generously give some hints: "Hospital", "Infectious diseases"
- Attach the requirement for Ampicillin trihydrate in USP 32 or Farmakope Indonesia 4 and sumarize the data that useful in this experiment. This experiment also useful for quality control for the antibiotic. In quality control of course there are quality standard. USP and Farmakope Indonesia provide the standard of Ampicillin trihydrate potency. You can access USP 32 or Farmakope Indonesia IV in our library if you don't have those books (Well, I belive you don't have them :P so stop by to our library). Then conclude whether the sample fulfil the requirement or not.
- For the procedure, please write exactly the same as what did you do at the experiment day. How much you weigh to make the main standard, how to make S1-S5, what is the concentration S1-S5, the lab apparatus that you used, how long was the pre-incubation, how long was the incubation, etc.
As you are international students that use English as the report language, please don't take any advantage of it by only copy-pasting english internet article to your report.. please rewrite with your own words the article that you think correlate with your report.
About the references:
- Laboratory guidance, lecture notes, blog posts can't be used as reference.
- Please write the references in right way, especially for references from internet article. You can check here how to write references properly.-
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